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p116r957 for  (Addgene inc)


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    Structured Review

    Addgene inc p116r957 for
    P116r957 For, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/p116r957+for/pBS+cScx+3'UTR+(CT%23485)+(Plasmid+%2313957)/ppr0548132-301-36-65
    Average 93 stars, based on 3 article reviews
    p116r957 for - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Cloning:

    Article Title: Immunodominant protein P116 from M. pneumoniae transports cholesterol and essential lipids
    Article Snippet: Hybridomas were selected in complete RPMI supplemented with HAT media and BM-Condimed (Sigma Aldrich, St. Louis, USA). .. Cloning, expression, and puri cation of P116 constructs Regions corresponding to the MPN213 gene from M. pneumoniae were ampli ed from synthetic clones using different primers for each construct: P116F30 and P116R957 for P116(30–957); P116F13 and P116R957 for P116(13–957); P116F212 and P116R862 for P116(212–862); and P116W681 to introduce mutation W681A PCR fragments were cloned into the expression vector pOPINE (gift from Ray Owens; plasmid #26043, Addgene, Watertown, USA) to generate constructs, with a C-terminal His-tag. .. Recombinant proteins were obtained after expression at 22°C in B834 (DE3) cells (Merck , Darmstadt, Germany), upon induction with 0.6 mM IPTG at 0.8 OD600.

    Expressing:

    Article Title: Immunodominant protein P116 from M. pneumoniae transports cholesterol and essential lipids
    Article Snippet: Hybridomas were selected in complete RPMI supplemented with HAT media and BM-Condimed (Sigma Aldrich, St. Louis, USA). .. Cloning, expression, and puri cation of P116 constructs Regions corresponding to the MPN213 gene from M. pneumoniae were ampli ed from synthetic clones using different primers for each construct: P116F30 and P116R957 for P116(30–957); P116F13 and P116R957 for P116(13–957); P116F212 and P116R862 for P116(212–862); and P116W681 to introduce mutation W681A PCR fragments were cloned into the expression vector pOPINE (gift from Ray Owens; plasmid #26043, Addgene, Watertown, USA) to generate constructs, with a C-terminal His-tag. .. Recombinant proteins were obtained after expression at 22°C in B834 (DE3) cells (Merck , Darmstadt, Germany), upon induction with 0.6 mM IPTG at 0.8 OD600.

    Construct:

    Article Title: Immunodominant protein P116 from M. pneumoniae transports cholesterol and essential lipids
    Article Snippet: Hybridomas were selected in complete RPMI supplemented with HAT media and BM-Condimed (Sigma Aldrich, St. Louis, USA). .. Cloning, expression, and puri cation of P116 constructs Regions corresponding to the MPN213 gene from M. pneumoniae were ampli ed from synthetic clones using different primers for each construct: P116F30 and P116R957 for P116(30–957); P116F13 and P116R957 for P116(13–957); P116F212 and P116R862 for P116(212–862); and P116W681 to introduce mutation W681A PCR fragments were cloned into the expression vector pOPINE (gift from Ray Owens; plasmid #26043, Addgene, Watertown, USA) to generate constructs, with a C-terminal His-tag. .. Recombinant proteins were obtained after expression at 22°C in B834 (DE3) cells (Merck , Darmstadt, Germany), upon induction with 0.6 mM IPTG at 0.8 OD600.

    Clone Assay:

    Article Title: Immunodominant protein P116 from M. pneumoniae transports cholesterol and essential lipids
    Article Snippet: Hybridomas were selected in complete RPMI supplemented with HAT media and BM-Condimed (Sigma Aldrich, St. Louis, USA). .. Cloning, expression, and puri cation of P116 constructs Regions corresponding to the MPN213 gene from M. pneumoniae were ampli ed from synthetic clones using different primers for each construct: P116F30 and P116R957 for P116(30–957); P116F13 and P116R957 for P116(13–957); P116F212 and P116R862 for P116(212–862); and P116W681 to introduce mutation W681A PCR fragments were cloned into the expression vector pOPINE (gift from Ray Owens; plasmid #26043, Addgene, Watertown, USA) to generate constructs, with a C-terminal His-tag. .. Recombinant proteins were obtained after expression at 22°C in B834 (DE3) cells (Merck , Darmstadt, Germany), upon induction with 0.6 mM IPTG at 0.8 OD600.

    Introduce:

    Article Title: Immunodominant protein P116 from M. pneumoniae transports cholesterol and essential lipids
    Article Snippet: Hybridomas were selected in complete RPMI supplemented with HAT media and BM-Condimed (Sigma Aldrich, St. Louis, USA). .. Cloning, expression, and puri cation of P116 constructs Regions corresponding to the MPN213 gene from M. pneumoniae were ampli ed from synthetic clones using different primers for each construct: P116F30 and P116R957 for P116(30–957); P116F13 and P116R957 for P116(13–957); P116F212 and P116R862 for P116(212–862); and P116W681 to introduce mutation W681A PCR fragments were cloned into the expression vector pOPINE (gift from Ray Owens; plasmid #26043, Addgene, Watertown, USA) to generate constructs, with a C-terminal His-tag. .. Recombinant proteins were obtained after expression at 22°C in B834 (DE3) cells (Merck , Darmstadt, Germany), upon induction with 0.6 mM IPTG at 0.8 OD600.

    Mutagenesis:

    Article Title: Immunodominant protein P116 from M. pneumoniae transports cholesterol and essential lipids
    Article Snippet: Hybridomas were selected in complete RPMI supplemented with HAT media and BM-Condimed (Sigma Aldrich, St. Louis, USA). .. Cloning, expression, and puri cation of P116 constructs Regions corresponding to the MPN213 gene from M. pneumoniae were ampli ed from synthetic clones using different primers for each construct: P116F30 and P116R957 for P116(30–957); P116F13 and P116R957 for P116(13–957); P116F212 and P116R862 for P116(212–862); and P116W681 to introduce mutation W681A PCR fragments were cloned into the expression vector pOPINE (gift from Ray Owens; plasmid #26043, Addgene, Watertown, USA) to generate constructs, with a C-terminal His-tag. .. Recombinant proteins were obtained after expression at 22°C in B834 (DE3) cells (Merck , Darmstadt, Germany), upon induction with 0.6 mM IPTG at 0.8 OD600.

    Polymerase Chain Reaction:

    Article Title: Immunodominant protein P116 from M. pneumoniae transports cholesterol and essential lipids
    Article Snippet: Hybridomas were selected in complete RPMI supplemented with HAT media and BM-Condimed (Sigma Aldrich, St. Louis, USA). .. Cloning, expression, and puri cation of P116 constructs Regions corresponding to the MPN213 gene from M. pneumoniae were ampli ed from synthetic clones using different primers for each construct: P116F30 and P116R957 for P116(30–957); P116F13 and P116R957 for P116(13–957); P116F212 and P116R862 for P116(212–862); and P116W681 to introduce mutation W681A PCR fragments were cloned into the expression vector pOPINE (gift from Ray Owens; plasmid #26043, Addgene, Watertown, USA) to generate constructs, with a C-terminal His-tag. .. Recombinant proteins were obtained after expression at 22°C in B834 (DE3) cells (Merck , Darmstadt, Germany), upon induction with 0.6 mM IPTG at 0.8 OD600.

    Plasmid Preparation:

    Article Title: Immunodominant protein P116 from M. pneumoniae transports cholesterol and essential lipids
    Article Snippet: Hybridomas were selected in complete RPMI supplemented with HAT media and BM-Condimed (Sigma Aldrich, St. Louis, USA). .. Cloning, expression, and puri cation of P116 constructs Regions corresponding to the MPN213 gene from M. pneumoniae were ampli ed from synthetic clones using different primers for each construct: P116F30 and P116R957 for P116(30–957); P116F13 and P116R957 for P116(13–957); P116F212 and P116R862 for P116(212–862); and P116W681 to introduce mutation W681A PCR fragments were cloned into the expression vector pOPINE (gift from Ray Owens; plasmid #26043, Addgene, Watertown, USA) to generate constructs, with a C-terminal His-tag. .. Recombinant proteins were obtained after expression at 22°C in B834 (DE3) cells (Merck , Darmstadt, Germany), upon induction with 0.6 mM IPTG at 0.8 OD600.



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    p116r957 for  (Addgene inc)
    93
    Addgene inc p116r957 for
    P116r957 For, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/p116r957+for/pBS+cScx+3'UTR+(CT%23485)+(Plasmid+%2313957)/ppr0548132-301-36-65
    Average 93 stars, based on 1 article reviews
    p116r957 for - by Bioz Stars, 2026-09
    93/100 stars
    		  Buy from Supplier

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